CRISPR/Cas9 gene editing of Aedes aegypti This protocol was originally published as a bench manual to accompany Kistler, Vosshall, and Matthews (2015), and is updated to reflect our current methodology for generating mutations in Aedes aegypti using CRISPR/Cas9.

piggyBac-mediated transformation of Aedes aegypti This protocol describes how to generate mRNA for piggyBac transposase and provides guidance on injection mixes for efficient transposon-mediated transformation of Aedes aegypti.